Recombinant:Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. Luminex® microspheres were coupled with recombinant human TIM-3 Fc (R&D Systems, Cat#2365-TM), recombinant human TIM-3 His (Sino Biological, Cat#10390-H08H), recombinant cynomolgus TIM-3 Fc (R&D Systems, Cat#7914-TM), recombinant human TIM-1 His (R&D Systems, Cat1750-TM), or recombinant human TIM-4 His (R&D, Cat#2929-TM), via amine coupling with the COOH bead surface. .. Purified pab2085, pab2088, and an IgG1 isotype control antibody were diluted in assay buffer (Roche, Cat#11112589001) to 10 ng/ml, 100 ng/ml, and 1000 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. LUMINEX® microspheres were coupled with recombinant human TIM-3 His (Sino Biological, #10390-H08H), recombinant cynomolgus TIM-3 Fc (Sino Biological, #90312-CO2H), recombinant mouse TIM-3 Fc (R&D Systems, #1529-TM), recombinant human TIM-1 His (R&D Systems, #1750-TM), recombinant human TIM-4 His (R&D, #2929-TM), recombinant human OX40 His (Sino Biological, #10481-H08H), recombinant human GITR Fc (R&D Systems, #689-GR), recombinant human DR3 Fc (R&D Systems, #943-D3), and recombinant human CD137 Fc (in house produced material), via amine coupling with the COOH bead surface. .. Purified pab2188w (IgG1 N297A), AM-2 (IgG1 N297A), AM-6 (IgG1 N297A), and an IgG1 N297A isotype control antibody were diluted in assay buffer (Roche 11112589001) to a dose titration from 10000 ng/ml to 0.1 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. LUMINEX® microspheres were coupled with recombinant human TIM-3 His (Sino Biological, #10390-H08H), recombinant cynomolgus TIM-3 Fc (Sino Biological, #90312-CO2H), recombinant mouse TIM-3 Fc (R&D Systems, #1529-TM), recombinant human TIM-1 His (R&D Systems, #1750-TM), recombinant human TIM-4 His (R&D, #2929-TM), recombinant human OX40 His (Sino Biological, #10481-H08H), recombinant human GITR Fc (R&D Systems, #689-GR), recombinant human DR3 Fc (R&D Systems, #943-D3), and recombinant human CD137 Fc (in house produced material), via amine coupling with the COOH bead surface. .. Purified pab2188w (IgG1 N297A), AM-2 (IgG1 N297A), AM-6 (IgG1 N297A), and an IgG1 N297A isotype control antibody were diluted in assay buffer (Roche 11112589001) to a dose titration from 10000 ng/ml to 0.1 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. LUMINEX® microspheres were coupled with recombinant human TIM-3 Fc (R&D Systems, Cat #2365-TM), recombinant human TIM-3 His (Sino Biological, Cat #10390-H08H), recombinant cynomolgus TIM-3 Fc (R&D Systems, Cat #7914-TM), recombinant human TIM-1 His (R&D Systems, Cat #1750-TM), or recombinant human TIM-4 His (R&D, Cat #2929-TM), via amine coupling with the COOH bead surface. .. Purified pab2085, pab2088, and an IgG1 isotype control antibody were diluted in assay buffer (Roche, Cat #11112589001) to 10 ng/ml, 100 ng/ml, and 1000 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. Luminex® microspheres were coupled with recombinant human TIM-3 His (Sino Biological, #10390-H08H), recombinant cynomolgus TIM-3 Fc (Sino Biological, #90312-C02H), recombinant mouse TIM-3 Fc (R&D Systems, #1529-TM), recombinant human TIM-1 His (R&D Systems, #1750-TM), recombinant human TIM-4 His (R&D, #2929-TM), recombinant human OX40 His (Sino Biological, #10481-H08H), recombinant human GITR Fc (R&D Systems, #689-GR), recombinant human DR3 Fc (R&D Systems, #943-D3), and recombinant human CD137 Fc (in house produced material), via amine coupling with the COOH bead surface. .. Purified pab2188w (IgG1 N297A), AM-2 (IgG1 N297A), AM-6 (IgG1 N297A), and an IgG1 N297A isotype control antibody were diluted in assay buffer (Roche 11112589001) to a dose titration from 10000 ng/ml to 0.1 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. Luminex® microspheres were coupled with recombinant human TIM-3 Fc (R&D Systems, Cat #2365-TM), recombinant human TIM-3 His (Sino Biological, Cat #10390-H08H), recombinant cynomolgus TIM-3 Fc (R&D Systems, Cat #7914-TM), recombinant human TIM-1 His (R&D Systems, Cat #1750-TM), or recombinant human TIM-4 His (R&D, Cat #2929-TM), via amine coupling with the COOH bead surface. .. Purified pab2085, pab2088, and an IgG1 isotype control antibody were diluted in assay buffer (Roche, Cat #11112589001) to 10 ng/ml, 100 ng/ml, and 1000 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. Luminex® microspheres were coupled with recombinant human TIM-3 His (Sino Biological, #10390-H08H), recombinant cynomolgus TIM-3 Fc (Sino Biological, #90312-C02H), recombinant mouse TIM-3 Fc (R&D Systems, #1529-TM), recombinant human TIM-1 His (R&D Systems, #1750-TM), recombinant human TIM-4 His (R&D, #2929-TM), recombinant human OX40 His (Sino Biological, #10481-H08H), recombinant human GITR Fc (R&D Systems, #689-GR), recombinant human DR3 Fc (R&D Systems, #943-D3), and recombinant human CD137 Fc (in house produced material), via amine coupling with the COOH bead surface. .. Purified pab2188w (IgG1 N297A), AM-2 (IgG1 N297A), AM-6 (IgG1 N297A), and an IgG1 N297A isotype control antibody were diluted in assay buffer (Roche 11112589001) to a dose titration from 10000 ng/ml to 0.1 ng/ml.
Produced:Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. LUMINEX® microspheres were coupled with recombinant human TIM-3 His (Sino Biological, #10390-H08H), recombinant cynomolgus TIM-3 Fc (Sino Biological, #90312-CO2H), recombinant mouse TIM-3 Fc (R&D Systems, #1529-TM), recombinant human TIM-1 His (R&D Systems, #1750-TM), recombinant human TIM-4 His (R&D, #2929-TM), recombinant human OX40 His (Sino Biological, #10481-H08H), recombinant human GITR Fc (R&D Systems, #689-GR), recombinant human DR3 Fc (R&D Systems, #943-D3), and recombinant human CD137 Fc (in house produced material), via amine coupling with the COOH bead surface. .. Purified pab2188w (IgG1 N297A), AM-2 (IgG1 N297A), AM-6 (IgG1 N297A), and an IgG1 N297A isotype control antibody were diluted in assay buffer (Roche 11112589001) to a dose titration from 10000 ng/ml to 0.1 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. LUMINEX® microspheres were coupled with recombinant human TIM-3 His (Sino Biological, #10390-H08H), recombinant cynomolgus TIM-3 Fc (Sino Biological, #90312-CO2H), recombinant mouse TIM-3 Fc (R&D Systems, #1529-TM), recombinant human TIM-1 His (R&D Systems, #1750-TM), recombinant human TIM-4 His (R&D, #2929-TM), recombinant human OX40 His (Sino Biological, #10481-H08H), recombinant human GITR Fc (R&D Systems, #689-GR), recombinant human DR3 Fc (R&D Systems, #943-D3), and recombinant human CD137 Fc (in house produced material), via amine coupling with the COOH bead surface. .. Purified pab2188w (IgG1 N297A), AM-2 (IgG1 N297A), AM-6 (IgG1 N297A), and an IgG1 N297A isotype control antibody were diluted in assay buffer (Roche 11112589001) to a dose titration from 10000 ng/ml to 0.1 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. Luminex® microspheres were coupled with recombinant human TIM-3 His (Sino Biological, #10390-H08H), recombinant cynomolgus TIM-3 Fc (Sino Biological, #90312-C02H), recombinant mouse TIM-3 Fc (R&D Systems, #1529-TM), recombinant human TIM-1 His (R&D Systems, #1750-TM), recombinant human TIM-4 His (R&D, #2929-TM), recombinant human OX40 His (Sino Biological, #10481-H08H), recombinant human GITR Fc (R&D Systems, #689-GR), recombinant human DR3 Fc (R&D Systems, #943-D3), and recombinant human CD137 Fc (in house produced material), via amine coupling with the COOH bead surface. .. Purified pab2188w (IgG1 N297A), AM-2 (IgG1 N297A), AM-6 (IgG1 N297A), and an IgG1 N297A isotype control antibody were diluted in assay buffer (Roche 11112589001) to a dose titration from 10000 ng/ml to 0.1 ng/ml.
Article Title: Anti-TIM-3 antibodies and methods of use thereof
Article Snippet: .. Luminex® microspheres were coupled with recombinant human TIM-3 His (Sino Biological, #10390-H08H), recombinant cynomolgus TIM-3 Fc (Sino Biological, #90312-C02H), recombinant mouse TIM-3 Fc (R&D Systems, #1529-TM), recombinant human TIM-1 His (R&D Systems, #1750-TM), recombinant human TIM-4 His (R&D, #2929-TM), recombinant human OX40 His (Sino Biological, #10481-H08H), recombinant human GITR Fc (R&D Systems, #689-GR), recombinant human DR3 Fc (R&D Systems, #943-D3), and recombinant human CD137 Fc (in house produced material), via amine coupling with the COOH bead surface. .. Purified pab2188w (IgG1 N297A), AM-2 (IgG1 N297A), AM-6 (IgG1 N297A), and an IgG1 N297A isotype control antibody were diluted in assay buffer (Roche 11112589001) to a dose titration from 10000 ng/ml to 0.1 ng/ml.
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